The PHP TAC is equipped with a Leica Bond Rx and an Aperio Versa whole tissue–slide imaging system. These instruments enable MSU researchers to implement a fully automated pipeline from tissue slide staining to image analysis. These combined technologies enable co-detection of up to 8 markers in a single tissue section. This innovative platform enables detailed in situ molecular characterization of diseased tissues with translational implications for personalized and precision medicine.
Bond Rx is a fully automated open system developed by Leica as a complementary platform to their FDA-approved clinical lockdown Bond-MAX staining station. Bond Rx is an ideal instrument in a translational research setting to develop and implement multiplex tissue-slide based assays for investigational applications and potential diagnostic applications (optimized protocols can be lockdown in Bond-MAX system for FDA approval). Hundreds of steps can be programmed for a single protocol which enables the co-detection of a large number of markers (3-4 with chromogenic stains and up to 8 with fluorescent stains). A single run with co-detection of up to 8 markers (any combination of DNA, RNA, glycans, and/or protein) is technically feasible and within performance indications of the instrument.
VERSA is a Brightfield, Fluorescence, and FISH Digital Pathology whole tissue–slide scanner. The Aperio VERSA is ideal for scanning multiplex whole tissue–slides of up to 8 fluorescent channels with customizable dichroic filters at any magnification from 5x to 63x for the whole slide. A unique feature of the VERSA imaging system is the ability to navigate, annotate, and analyze any field or all fields of the whole tissue section within is original context in a single file image using Aperio ImageScope software. The VERSA imaging system offers remote access and telepathology options. The VERSA system includes an analysis workstation and software tools with specialized algorithms. The Aperio Cellular IF Algorithm is used to quantify location, area, intensity, and colocalization of up to 8 fluorescence-labeled markers within same cell or cellular compartment. The Aperio TMALab is used to recognize and associate each tissue core with the appropriate map position for easy management and analysis.